不同处理方法对受体牛同期发情效果的比较研究

[目的]为了进一步促进人工授精技术广泛应用以及提高胚胎移植效率，为酒泉本地胚胎移植选择高效的同期发情方法。[方法]本试验分别采用PRID＋PGF2α法和PGF2α＋PGF2α法对胚胎移植受体牛做同期友情处理。[结果]表明：两种方法处理受体牛，其同期发情率和移植妊娠率之间差异无统计意义（P〉0．05），但是用PRID＋PGF2α法处理受体牛同期发情率和移植妊娠率均高于PGF2α＋PGF2α法。[结论]在奶牛养殖及生产上，在考虑移植成本和生产效益的情况下，在农区大面积对牛只进行同期发情技术处理，应优选PGF2α＋PGF2α法处理受体牛。     

Associations of Gonadotropin-Releasing Hormone Receptor (GnRHR) and Neuropeptide Y (NPY) Genes'''' Polymorphisms with Egg-Laying Traits in Wenchang Chicken

Single nucleotide polymorphisms (SNP) of chicken gonadotropin-releasing hormone receptor (GnRHR) and neuropeptide Y (NPY) were selected to identify the genotypes of Wenchang (Chinese indigenous breed) chicken with restricton fragment length polymorphisms. The associations of the SNPs with the total egg production (NE), average days of continual laying (ADCL), and number of double-yolked eggs (DYE) traits were analyzed. The frequency of restriction enzyme A/a alleles in the population was for GnRHR 0.69 (Bpu1102 Ⅰ A) and 0.31 (Bpu1102 Ⅰ a) and for NPY 0.46 (Dra Ⅰ B) and 0.54 (Dra Ⅰ b). Trait data from a total of 120 hens, which were purebred introduced from Hainan Province, China from one generation were recorded. Two significant effects of genes' marker were found for GnRHR and number of eggs (dominant; t= 2.67, df= 116) and NPY and number of eggs (additive; t= 1.97, df= 116). The current research supports the effects of GnRHR and NPY genes on egg-laying traits of chickens.     

孕酮处理对蛋鸡蛋壳钙化过程中CaBP-d28k基因表达的影响

实验旨在研究孕酮对蛋鸡CaBP-d28k基因表达的影响。对产蛋高峰期蛋鸡进行孕酮和孕酮受体拮抗剂(RU-486)处理,利用实时荧光定量PCR技术(QRT-PCR),测定在蛋壳形成过程中输卵管子宫部钙结合蛋白(CaBP-d28k)、孕酮受体(PGR)的表达量;同时对血液中钙离子和孕酮浓度变化进行测定。结果表明:孕酮处理后,血液中钙离子浓度显著降低(P<0.05),输卵管子宫部CaBP-d28k表达量也显著降低(P<0.05);孕酮受体拮抗剂处理后,血液中钙离子浓度显著升高(P<0.05),输卵管子宫部CaBP-d28k表达量极显著升高(P<0.01)。结果提示,在蛋壳钙化过程中,孕酮对CaBP-d28k表达和钙离子转运起负调控作用。     

死亡受体6的作用机制及其生物学功能

死亡受体6(death receptor 6,DR6)是肿瘤坏死因子受体超家族中的成员。DR6是Ⅰ型跨膜受体,拥有4个胞外半胱氨酸基序和1个胞内死亡结构域。在人的大多数组织表达中,已经发现其在心、脑、胎盘、胰脏、胸腺、淋巴结和几个非淋巴癌细胞系中表达。DR6可以和TRADD相互作用,激活JNK和NF-κB途径。DR6能下调T、B细胞的增殖和活化,诱导神经细胞的凋亡等。     

温度、丙烯和1-MCP对西洋梨果实乙烯合成和乙烯受体ETR1同源基因表达的影响

研究了贮藏温度、丙烯和 1 MCP (1-甲基环丙烯 )处理对帕斯—卡桑 (Passe -Crassane)西洋梨果实成熟的影响。结果表明 :低温可促进果实的乙烯合成 ,未经低温处理的果实 ,用丙烯处理 ,不能正常合成乙烯。丙烯促进乙烯合成的效应随冷藏时间延长而增强。1 MCP处理可抑制冷藏果实的乙烯合成。定量PCR分析结果表明 ,果实中乙烯受体ETR1同源基因的表达不受低温的调节 ;但冷藏后升温或升温后用丙烯处理时 ,mRNA含量降低     

催乳素及其受体对乳腺发育研究进展

本文主要对催乳素受体的结构、作用机理、功能及其在不同时期的乳腺发育的不同作用进行的论述。总结了近几年来的有关研究进展,为进一步的研究工作提供参考资料。     

鸡白细胞介素-2研究进展

白细胞介素－2（interleukin-2,IL-2)主要是由激活的T淋巴细胞产生的一类重要的细胞因子，具有激活淋巴因子活化的杀伤细胞（LAK）和自然杀伤细胞，刺激T辅助细胞和自然杀伤细胞产生细胞因子和促进细胞因子的繁殖以及刺激γδT细胞在体外生长等功能，因此它是免疫学研究的热点。近年来鸡IL－2基因克隆成功，使鸡IL－2的研究及应用得到一定的发展，同时也使鸡IL－2作为免疫佐剂和构建新型的基因工程疫苗呈现出一定的应用前景，本文主要对鸡IL－2研究取得的进展作一综述。     

Advance in receptor type protein tyrosine phosphatase Q

Receptor type protein tyrosine phosphatase Q (PTPRQ) is an unusual protein tyrosine phosphatase that has intrinsic dephosphorylating activity for various phosphatidylinositiol and phospho-tyrosine substrates, especially the phosphatidylinositol activity. Recent data show that PTPRQ has an important role in various biological processes and is associated with some diseases. In this article, the structure and function of PTPRQ and the relationship between PTPRQ and diseases were briefly summarized.     

Role of PPARα/PGC-1α in doxorubicin induced mouse dilated cardio-myopathy

AIM: To investigate the changes of peroxisome proliferator-activated receptors (PPAR)α/peroxisome proliferator activated receptor coactivator 1 alpha (PGC-1α) in doxorubicin (DOX) induced dilated cardiomyopathy (DCM) and its effect on the energy metabolism and myocardial function in mice. METHODS: Forty mice were randomly divided into 4 groups: control group, DOX group, PPARα inhibitor group and PPARα agonist group. The DCM model was established by injection of DOX. The protein levels of PPARα/PGC-1α were detected. The PPARα inhibitor and PPARα agonist were used 2 weeks beforeinjection of DOX. The contents of adenine acid and phosphocreatine (Pcr) in the mitochondria were measured by high-performance liquid chromatography (HPLC). The ANT activity was analyzed by the atractyloside-inhibitor stop technique. The changes of the echocardiography and hemodynamics were also observed. RESULTS: DOX induced DCM model was successfully established. The protein levels of PPARα and PGC-1α in control group were significantly higher than those in DOX group (P<0.05). Both of the high-energy phosphate contents and the transport activity of ANT were decreased in DOX group (P<0.05), and the hemodynamic parameters were disordered (P<0.01). Compared with DOX group, PPARα inhibitor pre-treatment significantly reduced the PPARα/PGC-1α expression. Meanwhile, high-energy phosphate contents in the mitochondria and the ANT transport activity of the mitochondria decreased, as well as the left ventricular function (P<0.05). On the other hand, PPARα agonist significantly increased the expression of PPARα and PGC-1α, and improved the transport activity of ANT. In addition, the hemodynamic parameters were ameliorated, but the high-energy phosphate contents of the mitochondria did not significantly change. CONCLUSION: PPARα/PGC-1α plays an important role in the regulation of ANT transport activity in dilated cardiomyopathy induced by DOX, and the activation of PPARα/PGC-1α has protective effects on the DCM induced by DOX.